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L.lactis HCP ELISA kit
This kit has been specifically developed and validated for use in detecting L.lactis HCPs as contaminants in products produced by recombinant expression in Lactococcus lactis. The antibodies used in this kit were generated against and affinity purified from HCPs obtained from the spent culture media of a null cell line. Our assay validation has determined that the LOD for this kit is ~0.125ng/mL.
Host Cells used for recombinant expression are complex systems containing hundreds to thousands of host cell proteins (HCPs) that can contaminate biopharmaceutical products. Failure to sufficiently remove contaminants early in drug development can result in reduced drug efficacy or adverse patient reactions.
Regulations require HCPs be reduced to the lowest levels possible. Our generic ELISA kits are useful in both process development and process validation. Using these kits in the earliest phase of product development can provide relevant and specific data directing process improvement and decreasing the levels of HCP contamination.
In quality control applications, subject to thorough validation and qualification by each laboratory, these kits may also be useful for lot release testing.
相關(guān)產(chǎn)品：
Product Catalog # Description
A549 HCP ELISA kit F230 Host cell protein assay, 96 tests
A549 HCP Western Blot Kit F235 Host cell protein assay, 5 mini blots
BHK HCP ELISA Kit F510 Host cell protein assay, 96 tests
Bovine IgG ELISA Kit F070 Bioprocess contaminant assay, 96 tests
Bovine Plasma Protein ELISA Kit F290 Bioprocess contaminant assay, 96 tests
BSA ELISA Kit F030 Bioprocess contaminant assay, 96 tests
Bovine Transferrin ELISA Kit F120 Bioprocess contaminant assay, 96 tests
CHO HCP ELISA Kit F015 Host cell protein assay, 96 tests
CHO HCP Western Blot Kit F060 Host cell protein assay, 5 mini blots
CHO-CM ELISA Kit CM015 Host cell protein assay, 96 tests
CHO-CM Western Blot Kit CM060 Host cell protein assay, 5 mini blots
CHO HCP ELISA Kit, 3G F550 Host cell protein assay, 96 tests
E. coli HCP ELISA Kit F410 Host cell protein assay, 96 tests
E. coli HCP Western Blot Kit F415 Host cell protein assay, 5 mini blots
Goat Casein HCP ELISA Kit F420 Host cell protein assay, 96 tests
Goat IgG ELISA Kit F210 Bioprocess contaminant assay, 96 tests
Total Goat Milk Proteins ELISA kit F240 Host cell protein assay, 96 tests
Total Goat Milk Proteins WB kit F245 Host cell protein assay, 5 mini blots
Human Albumin ELISA Kit F055 Bioprocess contaminant assay, 96 tests
Human IgA ELISA Kit F165 Bioprocess contaminant assay, 96 tests
Human IgG-total ELISA Kit F160 Bioprocess contaminant assay, 96 tests
Human IgM ELISA Kit F170 Bioprocess contaminant assay, 96 tests
HEK 293 HCP ELISA Kit F650 Host cell protein assay, 96 tests
Human Transferrin ELISA Kit F035N Bioprocess contaminant assay, 96 tests
Insect Cell SF9 HCP ELISA Kit F020 Host cell protein assay, 96 tests
Insect Cell SF9 Western Blot Kit F125 Host cell protein assay, 5 mini blots
Insulin ELISA Kit F040 Bioprocess contaminant assay, 96 tests
Lactococcus lactic HCP ELISA Kit F490 Host cell protein assay, 96 tests
Lactococcus lactic HCP WB Kit F495 Host cell protein assay, 5 mini blots
Mouse Total IgG ELISA Kit F049 Bioprocess contaminant assay, 96 tests
Mouse IgG1 ELISA Kit F045 Bioprocess contaminant assay, 96 tests
Mouse IgG2a ELISA Kit F046 Bioprocess contaminant assay, 96 tests
Mouse IgG2b ELISA Kit F047 Bioprocess contaminant assay, 96 tests
Mouse IgM ELISA Kit F090 Bioprocess contaminant assay, 96 tests
MRC5 HCP ELISA Kit F300 Host cell protein assay, 96 tests
NS/0 HCP ELISA kit F220 Host cell protein assay, 96 tests
NS/0 HCP Western Blot kit F225 Host cell protein assay, 5 mini blots
PER.C6 cell line HCP ELISA Kit F530 Host cell protein assay, 96 tests
Pichia pastoris HCP ELISA Kit F140 Host cell protein assay, 96 tests
P.pastoris HCP Western Blot Kit F145 Host cell protein assay, 5 mini blots
Pichia pastoris HCP ELISA Kit, 2nd Generation F640 Antibodies to glycosylation epitopes have been removed and is much more specific than the F140.
Protein A ELISA Kit F050 Bioprocess contaminant assay, 96 tests
Protein A ELISA for human IgG F050H Bioprocess contaminant assay, 96 tests
Protein A ELISA kit F400 Bioprocess contaminant assay, 96 tests
Ps. fluorescens HCP ELISA kit F450 Host cell protein assay, 96 tests
S. cerevisiae HCP ELISA Kit F135 Host cell protein assay, 96 tests
S. cerevisiae HCP Western Blot Kit F130 Western Blot assay, 5 mini blots
SP 2/0 HCP ELISA Kit F180 Host cell protein assay, 96 tests
SP 2/0 HCP Western Blot Kit F185 Western Blot assay, 5 mini blots
S.aureus HCP ELISA Kit F320 Host cell protein assay, 96 tests
Ultra-Sensitive Insulin ELISA Kit F280 Bioprocess contaminant assay, 96 tests
Vero cell HCP ELISA kit F500 Host cell protein assay, 96 tests
Polyclonal Antisera
A549 HCP A549 Goat, neat serum
A549 HCP A549-PA Goat, IgG fraction
A549 HCP A549-AF Goat, affinity purified
Bovine Plasma Proteins BPP04 Rabbit, neat serum
Bovine Plasma Proteins BPP04-PA Rabbit, IgG fraction
Bovine Plasma Proteins BPP04-AF Rabbit, affinity purified
BHK HCP BHK1107 Goat, neat serum
BHK HCP BHK1107-PA Goat, IgG fraction
BHK HCP BHK1107-AF Goat, affinity purified
Campylobacter species 9-25B-PA Rabbit, IgG fraction
Campylobacter species 9-61A Guinea Pig, neat serum
Chlamydia species 8-29A Rabbit, neat serum
Chlamydia species 8-29A-PA Rabbit, IgG fraction
Chlamydia species 8-48 Guinea Pig, neat serum
CHO HCP C0016 Goat, neat serum
CHO HCP C0016-PA Goat, IgG fraction
CHO HCP C0016-AF Goat, affinity purified
CHO-CM HCP M0016 Goat, neat serum
CHO-CM HCP M0016-PA Goat, IgG fraction
CHO-CM HCP M0016-AF Goat, affinity purified
CHO HCP 3G 3G-0016 Goat, neat serum
CHO HCP 3G 3G-0016PA Goat, IgG fraction
CHO HCP 3G 3G-0016AF Goat, affinity purified
Cytomegalovirus 8-21B Rabbit, neat serum
Cytomegalovirus 8-53A Guinea Pig, neat serum
E. coli HCP F117 Goat, neat serum
E. coli HCP F117-PA Goat, IgG fraction
E. coli HCP AP117 Goat, affinity purified
Haemophilus influenzae 8-21A Rabbit, neat serum
Haemophilus influenzae 8-21A-PA Rabbit, IgG fraction
Haemophilus influenzae 8-53B Guinea Pig, neat serum
Haemophilus influenzae 8-53B-PA Guinea Pig, IgG fraction
HEK 293 HCP HK293 Goat, neat serum
Herpes Simplex Virus 8-25A Rabbit, neat serum
Herpes Simplex Virus 8-25A-PA Rabbit, IgG fraction
Herpes Simplex Virus 8-40B Guinea Pig, neat serum
Klebsiella pneumoniae, Genus 9-01 Rabbit, neat serum
Lactococcus lactic HCP LL5557 Goat, neat serum
Lactococcus lactic HCP LL5557-PA Goat, IgG fraction
Lactococcus lactic HCP LL5557-AF Goat, affinity purified
MRC5 HCP MRC5 Goat, neat serum
MRC5 HCP MRC5-PA Goat, IgG fraction
MRC5 HCP MRC5-AF Goat, affinity purified
Mycobacterium genus 8-27B Rabbit, neat serum
Mycobacterium genus 1-04-PA Guinea Pig, IgG fraction
Neisseria meningitidis 8-24B Rabbit, neat serum
Neisseria meningitidis 8-36 Guinea Pig, neat serum
NS/0 HCP N9192 Goat, neat serum
NS/0 HCP N9192-PA Goat, IgG fraction
NS/0 HCP N9192-AF Goat, affinity purified
PER.C6 cell line HCP PC118 Goat, neat serum
PER.C6 cell line HCP PC118-PG Goat, IgG fraction
PER.C6 cell line HCP PC118-AF Goat, affinity purified
Pichia pastoris HCP PI-49 Goat, neat serum
Pichia pastoris HCP PI-49PA Goat, IgG fraction
Pichia pastoris HCP PI-49AF Goat, affinity purified
Pichia pastoris HCP PI-49AF-B Goat, affinity purified-biotinylated
Pichia pastoris HCP PI-49AF-AP Goat, affinity purified-alk. phosphatase
Pseudomonas fluorescens 7-04 Goat, neat serum
Pseudomonas fluorescens 7-04PA Goat, IgG fraction
Pseudomonas fluorescens 7-04AF Goat, affinity purified
Pseudomonas species 8-57 Guinea Pig, neat serum
Saccharomyces cerevisiae HCP 82-SC Goat, neat serum
Saccharomyces cerevisiae HCP 82SC-PA Goat, IgG fraction
Saccharomyces cerevisiae HCP 82SC-AF Goat, affinity purified
Shigella species 9-03-PA Rabbit, IgG fraction
Shigella species 9-04 Rabbit, neat serum
SF9 HCP 12-SF9 Rabbit, neat serum
SF9 HCP 12-SF9-PA Rabbit, IgG fraction
SF21 HCP 34-SF21 Rabbit, neat serum
SF21 HCP 34-SF21-PA Rabbit, IgG fraction
SP2/0 HCP SP110 Goat, neat serum
SP2/0 HCP SP110-PA Goat, IgG fraction
SP2/0 HCP SP110-AF Goat, affinity purified
SP2/0 HCP SP110AF-B Goat, affinity purified-biotinylated
SP2/0 HCP SP110AF-AP Goat, affinity purified-alk. phosphatase
S. aureus HCP SA8114 Goat, neat serum
S. aureus HCP SA8114-AF Goat, affinity purified
Streptococcus pneumoniae 8-31B Rabbit, neat serum
Streptococcus pneumoniae 8-42A Guinea Pig, neat serum
Streptococcus pneumoniae 8-43A-PA Guinea Pig, IgG fraction
Total Goat Milk Proteins 4042 Rabbit, neat serum
Total Goat Milk Proteins 4042-PA Rabbit, IgG fraction
Total Goat Milk Proteins 4042-AF Rabbit, affinity purified
Vero cell HCP VC807 Goat, neat serum
Vero cell HCP VC807-PA Goat, IgG fraction
Vero cell HCP VC807-AF Goat, affinity purified
Stab-ELISA-rÔ Enzyme Conjugate Diluent/Stabilizers
Alkaline Phosphatase I-045 500mL
Alkaline Phosphatase I-040 1000mL
Alk Phos w/ HAMA blocker I-045H 500mL
Alk Phos w/ HAMA blocker I-040H 1000mL
Horseradish Peroxidase I-035 500mL
Horseradish Peroxidase I-030 1000mL
HRP w/ HAMA blocker I-035H 500mL
HRP w/ HAMA blocker I-030H 1000mL
Liquid Substrates
PNPP Liquid Substrate F008 For use with Alk Phos in ELISA, 100mL
PNPP Liquid Substrate F10008 For use with Alk Phos in ELISA, 1L
TMB Liquid Substrate F027 For use with HRP in ELISA, 100mL
TMB Liquid Substrate F100027 For use with HRP in ELISA, 1L
TMB Liquid Substrate F129 For use with HRP in Western Blot, 100mL
TMB Liquid Substrate F100029 For use with HRP in Western Blot, 1L
BCIP/NBT Liquid Substrate F064 For use with Alk Phos in WB, 1 reagent, 100mL
BCIP/NBT Liquid Substrate F00064 For use with Alk Phos in WB, 1 reagent, 1L
Sample Dilution Buffers
Sample Dilution Buffer, 1L， 100mL, 500mL I028 I028-100 I028-500 For use with BHK, E.coli, CHO, CHO-CM, Human IgG's, HSA, hTransferrin, HEK 293, Insulin, L.lactic, Mouse IgG's, Pichia pastoris, S.cerevisiae, Protein A, Vero cell ELISA kits
A549 Sample Diluent, 100mL , 500mL F233A-100 F233A-500 For use with A549 HCP ELISA kit
BSA Sample Dilution Buffer, 1L , 100mL , 500mL F031A / F031A-100 / F031A-500 For use with BSA, Bovine IgG, Bovine Plasma Proteins and Bovine Transferrin ELISA kits
Goat IgG Dilution Buffer, 1L , 100mL , 500mL F213A /F213A-100 / F213A-500 For use with Goat IgG ELISA kit
Goat Milk Diluent Buffer, 1L , 100mL r, 500mL F243A /F243A-100 /F243A-500 For use with Goat Milk Proteins ELISA kit
NS/0 Sample Dilution Buffer, 1L ，100mL ，500mL F223A / F223A-100 /F223A-500 For use with NS/0 HCP ELISA kit
SP2/0 Sample Dilution Buffer, 1L , 100mL , 500mL F183A / F183A-100 / F183A-500 For use with SP2/0 HCP ELISA kit
Immunogenicity Products
Passive Coated Immunogenicity Kit IM10 Kit for immunogenicity testing
Biotinylated Antigen Coated Immunogenicity Kit IM20 Kit for immunogenicity testing
Biotinylation Kit IM30
Human Immunoglobulin Screening Kit IM40 Kit for immunogenicity testing
Human IgG Subclass Kit IM50 Kit for immunogenicity testing
Substrate Kit IM109 TMB & Stop Solution for ELISA, 50mL
Coating Buffer, 1L IM101 For immunogenicity testing
Conjugate Diluent, 1L IM103 For immunogenicity testing
Conjugate Diluent, 500mL IM103-500 For immunogenicity testing
Sample Diluent, 500mL IM104 For immunogenicity testing
FG Blocker, 1L IM107 For immunogenicity testing
BSA Antigen, 1mg/mL IM112 For immunogenicity testing
Bovine IgG Antigen, 1mg/mL IM113 For immunogenicity testing
Bovine Transferrin Antigen, 1mg/mL IM114 For immunogenicity testing
CHO HCP Antigen, 1mg/mL IM115 For immunogenicity testing
E. coli HCP Antigen, 1mg/mL IM116 For immunogenicity testing
Goat IgG Antigen, 1mg/mL IM117 For immunogenicity testing
HSA Antigen, 1mg/mL IM118 For immunogenicity testing
Human IgD Antigen, 1mg/mL IM141 For immunogenicity testing
Human Transferrin Antigen, 1mg/mL IM119 For immunogenicity testing
Insulin Antigen, 1mg/mL IM121 For immunogenicity testing
Mouse IgG Antigen, 1mg/mL IM122 For immunogenicity testing
NS/0 HCP Antigen, 1mg/mL IM123 For immunogenicity testing
SP2/0 HCP Antigen, 1mg/mL IM125 For immunogenicity testing
Gyros Kits-discontinued
CHO 3G HCP G550 For use on the Gyros xP workstation, 560 tests
CHO 3G HCP G550-1 For use on the Gyros xP workstation, 112 tests
E.coli HCP G410 For use on the Gyros xP workstation, 560 tests
E.coli HCP G410-1 For use on the Gyros xP workstation, 112 tests
Protein A G400 For use on the Gyros xP workstation, 560 tests
Protein A G400-1 For use on the Gyros xP workstation, 112 tests
DNA Kits
DNA Extracton in Wells D100W 96 tests in a deep well microtiter plate
DNA Extraction in Tubes D100T 50 tests in microfuge tubes
Residual CHO Host Cell kit in Wells D550W 96 tests in a deep well microtiter plate
Residual CHO Host Cell kit in Tubes D550T 50 tests in microfuge tubes
CHO Amplification kit in Wells D555W 96 tests in a deep well microtiter plate
CHO Amplification kit in Tubes D555T 50 tests in microfuge tubes
Residual Ecoli Host Cell kit in Wells D410W 96 tests in a deep well microtiter plate
Residual Ecoli Host Cell kit in Tubes D410T 50 tests in microfuge tubes
E.coli Amplification kit in Wells D415W 96 tests in a deep well microtiter plate
E.coli Amplification kit in Tubes D415T 50 tests in microfuge tubes
Residual NS/0 Host Cell kit in Wells D220W 96 tests in a deep well microtiter plate
Residual NS/0 Host Cell kit in Tubes D220T 50 tests in microfuge tubes
Miscellaneous Reagents & Components
Deep Well Extraction Plate w/Sealing Mat D102 5 plates
DNA Extraction Buffer for use in tubes D108 30mL
DNA Extraction Buffer for use in wells D105 30mL
DNA Sample Diluent D006-100 100mL
DNA Sample Diluent D006-500 500mL
DNA Sample Diluent D006-1000 1L
DNA TE Buffer, 1L D001 1L
CHO DNA Concentrate D552 10ug/mL, 1mL
E.coli DNA Concentrate D412 10ug/mL, 1mL
NS/0 DNA Concentrate D222 10ug/mL, 1mL
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